Coursework and practical plan for title "Investigate the effect of temperature on the action of the enzyme diastase on starch."

For my investigation, I plan to examine the effect temperature has on enzyme action involving the biological catalyst- diastase, and its substrate- starch. In lesson, I have conducted a preliminary experiment to give me an idea of what to expect and a chance to conduct the experiment before hand. From my preliminary work I believe that an equal amount of substrate (Starch) with an equal amount of enzyme (Diastase) should finish reacting at around 90-120 …

…is little I can do about this, however if it is an inhibitor, then it will reduce the speed of reaction once a sample has been taken. To overcome this problem, I will take a sample from the whole solution, and add iodine to the sample- this will reduce the impact it has on affecting the test- rather than add iodine to the whole solution and wait to see if and when it stops reacting.