Plasmid Fusion and PCR
Date Submitted: 10/16/2004 01:59:42
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think that the fact that the wells were glowing means that the PCR did work and that the glowing product in the wells is the product of the PCR experiment. This is because in the PCR machine all the DNA is separated and jumbled, the primer helps to form the only full DNA structure so when we added the yellow loading buffer it could only bond with the full DNA which was the tPA-25-ALU.
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